Anti-Mouse FGF R2 (IIIc)

Pricing & Details

Product No.F1082
Clone
133706
Protein
FGF R2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IIIc, Fibroblast Growth Factor Receptor 2
Isotype
Rat
IgG2a
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
F1082-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
F1082-100 µg
100 µg
$420.00
In stock
Max:
Min: 1
Step: 1
F1082-500 µg
500 µg
$1,025.00
In stock
Max:
Min: 1
Step: 1
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Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
NS0 and Sf 21-Derived Extracellular Domains of Recombinant Mouse FGF Receptor 2 Isoforms
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse FGF R2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse FGF R2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse FGF R2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse FGF R2 (at 3 ng/ml) is 0.1 - 0.4 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Fibroblast Growth Factor Receptor 2 (FGF R2) (Clone 133706) recognizes an epitope on Mouse FGF R2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody displays 100% cross-reactivity with IIIc isoforms of rhFGF R2 and no cross-reactivity with IIIb isoforms of rhFGF R2 or any isoforms of rhFGF R1, rmFGF R3 or rhFGF R4.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
General Western Blot Protocol

Formats Available

Prod No.
Description
F1082
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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