Anti-Mouse Galectin-9 (Clone RG9-1) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C3342
Clone
RG9-1
Protein
Galectin-9
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Galectin-9, RG9-1
Isotype
Rat
IgG2b κ
Applications
B
Prod No.
Size
Price
Avail.
Qty
Add to cart
C3342-1.0mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C3342-5.0mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C3342-25mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C3342-50mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C3342-100mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Recombinant mouse galectin-9
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
Purity
≥95% by SDS Page
≥98% monomer by analytical SEC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Other Applications Reported In Literature ?
B
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
RG9-1 activity is directed against mouse galectin-9.
Antigen Distribution
Galectin-9 is expressed by many cell types, including T cells, B cells, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, endothelial cells, and epithelial cells.
Background
Galectin-9 (Gal-9), a member of the galectin family of mammalian lectins, binds β-galactoside. Gal-9 is expressed by many cell types, including endothelial cells, the epithelium of the gastrointestinal tract, T cells, B cells, macrophages, and mast cells1. Gal-9 plays a significant role in innate and adaptive immunity and regulates excessive immunity by suppressing interleukin (IL)-17 producing effector T helper cells (Th)17 and Th1 as well as by augmenting Foxp3+ regulatory T cells (Treg). In addition, Gal-9 induces monocytic myeloid-derived suppressor cells (MDSCs), granulocytic MDSCs, and plasmacytoid dendritic cell-like macrophages. Gal-9 also suppresses B cell receptor signaling and is regulated by I-branching of N-glycans2. Additionally, Gal-9 stimulates the maturation of dendritic cells3.

Gal-9 is thought to function by binding to specific carbohydrate moieties in receptor molecules expressed on the surface of its target cells, including the T-cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3)1. Gal-9 is predominantly located in the cytoplasm but can also be secreted3. Gal-9 is expressed by a variety of tumor cells, plays a role in tumor immunity, and can suppress tumor metastasis by blocking adhesion to endothelium and extracellular matrices4. CD45, which regulates antigen receptor signaling, is a major glycoprotein receptor for Gal-9 on naive B cells2.

Antigen Details

Protein
Ligand/Receptor
Binds to β-galactosides and can serve as a ligand for TIM-3 (CD366)
Research Area
Immunology

References & Citations

1. Oomizu S, Arikawa T, Niki T, et al. PLoS One. 7(11):e48574. 2012.
2. Giovannone N, Liang J, Antonopoulos A, et al. Nat Commun. 9: 3287 (2018).
3. Yang R, Rabinovich G, Liu F. Expert Rev Mol Med. 10, E17. 2008.
4. Nobumoto A, Nagahara K, Oomizu S, et al. Glycobiology. 18(9):735-744. 2008
5. de Mingo Pulido Á, Gardner A, et al. Cancer Cell. Jan 8;33(1):60-74.e6. 2018.
6. Daley D, Zambirinis CP, Seifert L, et al. Cell. 8;166(6):1485-1499.e15. 2016.
7. Dolina JS, Braciale TJ, Hahn YS. Hepatology. Apr;59(4):1351-1365. 2014.

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.