Anti-Mouse GFRα-2

Anti-Mouse GFRα-2

Product No.: G660

[product_table name="All Top" skus="G660"]

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GFR alpha -2
Product Type
Polyclonal Antibody
Alternate Names
Glial Cell Line-Derived Neurotropic Factor Receptor Alpha 2, GDNFRB, NRTNR-Alpha, NTNRA, RETL2, TRNR2

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Product Size
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Antibody Details

Product Details

Reactive Species
Host Species
Purified Recombinant Human/Mouse GFRα-2 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human/Mouse GFRα-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human/Mouse GFRα-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Blocking: Blockade of receptor-ligand interaction - Approximately 1-3 μg/ml of this antibody will block 50% of the binding of 2 ng/ml recombinant human GDNF to immobilized recombinant mouse GFRα-2/Fc Chimera (100 μl of a 1 μg/ml solution was coated in each well) in a functional ELISA assay.
Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Goat Anti-Human/Mouse Glial Cell-Line Derived Neurotrophic Factor Receptor Alpha 2 (GFRα-2) recognizes Human/Mouse GFRα-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Lewin, GR. et al. (2002) J Physiol. 545: 43
2. Mulligan, LM. et al. (2001) Human Genetics 108: 409
3. Herzig, KH. et al. (2005) Diabetes 54: 1324
General Western Blot Protocol
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Prod No.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.