Anti-Mouse H-2Kd Dd,q,u – DyLight® 488

Anti-Mouse H-2Kd Dd,q,u – DyLight® 488

Product No.: H1555

[product_table name="All Top" skus="H1555"]

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Clone
A5A1-Do4
Target
I H-2Kd Dd
q
u
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG3 κ
Applications
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Immunogen
Purified Recombinant Mouse I H-2Kd Dd,q,u (>98%)
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (493 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone A5A1-Do4 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Specificity
Mouse Anti-Mouse I H-2Kd Dd,q,u (Clone A5A1-Do4) recognizes an epitope on Mouse I H-2Kd Dd,q,u. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Present on 100% of T-cells, B-cells, erythrocytes, macrophages, endothelial and epithelial tissue. Positive with this antibody are strains of independent haplotypes H-2KdDd,q,u. Positive are intra-H-2 recombinants carrying alleles Kd, Dd, Ld, Dq. Strains carrying all other alleles are negative.

Antigen Details

Ligand/Receptor
CD3/TCR, CD8
Research Area
Immunology
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Innate Immunity

References & Citations

1.Hauptfeld, V. (1984) Immunogenetics 19:169
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.