Anti-Mouse H-2Kd Dd,q,u – Purified

Anti-Mouse H-2Kd Dd,q,u – Purified

Product No.: H162

[product_table name="All Top" skus="H162"]

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Clone
A5A1-Do4
Target
I H-2Kd Dd
q
u
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG3 κ
Applications
FC
,
IHC FF
,
IP
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone A5A1-Do4 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IP
WB
IHC FF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Mouse I H-2Kd Dd,q,u (Clone Do4) recognizes Mouse I H-2Kd Dd,q,u. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
Present on 100% of T-cells, B-cells, erythrocytes, macrophages, endothelial and epithelial tissue. Positive with this antibody are strains of independent haplotypes H-2KdDd,q,u. Positive are intra-H-2 recombinants carrying alleles Kd, Dd, Ld, Dq. Strains carrying all other alleles are negative.

Antigen Details

References & Citations

Hauptfeld, V. (1984) Immunogenetics 19:169
Flow Cytometry
IHC FF
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.