Anti-Mouse/Human CD45R (Clone RA3-6B2) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse/Human CD45R (Clone RA3-6B2) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C517

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Clone
RA3-6B2
Target
CD45R
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
B220
Isotype
IgG2a κ
Applications
IHC FF
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PhenoCycler®

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CODEX® Details

Validation Notes
Clone RA3-6B2 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse/Human
Host Species
Rat
Concentration
0.5 mg/ml
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD45R (Clone RA3-6B2) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893574
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse/Human CD45R (Clone RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.
Antigen Distribution
The CD45R antigen is present on mouse B-cells, B-cell precursors and lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC restricted CTL).
Background
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase suppression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.

Antigen Details

Protein
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
PubMed
NCBI Gene Bank ID

References & Citations

1. Coffman, B. et al. (1982) Immunological Rev. 69:5
2. Zuhair, K. et al. (1993) J. Immunol. 150:17
3. Asensi, V. et al. (1989) Immunology 68:204
IHC
PhenoCycler®
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.