Anti-Mouse IFNß – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.B659
Clone
HDß-4A7
Protein
IFNβ
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Clone HDb-4A7,
Isotype
Mouse
IgG2a
Applications
B
,
FA
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
B659-1.0 mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
B659-5.0 mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
B659-25 mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
B659-50 mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
B659-100 mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Mouse
Immunogen
Plasmid DNA encoding murine IFNb
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Other Applications Reported In Literature ?
FA
B
N
WB


In Vitro Activity of HDß-4A7 - Inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). Blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. Neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV.
In Vivo Activity of HDß-4A7 circulates with a half-life of ~ 14 days. Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Mouse IFNß recognizes an epitope on Mouse IFNß & binds specifically to murine IFNb and does not bind murine IFNg or several different IFNa species HDb-4A7 binds both recombinant and natural forms of IFNb. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Apoptosis
.
Cell Biology
.
Cell Death
.
Signal Transduction
.
Tumor Suppressors

References & Citations

1. Oldstone, Michael B. A. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. PubMed
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.