Anti-Mouse IFNAR-1 – PE

Product No.: I-1033

[product_table name="All Top" skus="I-1033"]

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Clone
MAR1-5A3
Target
IFNAR1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD118, Ifar, Ifnar, Ifrc, INF-a receptor, Interferon-α/β receptor α chain precursor
Isotype
IgG1
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Immunogen
This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone MAR1-5A3 PE conjugated antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone MAR1-5A3 recognizes an epitope on mouse IFNAR1.
Antigen Distribution
IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
Background
IFNAR1 is a type I membrane protein, that in conjunction with IFNAR2, makes up the heterodimeric receptor that binds all type I IFNs, which includes IFN α and β. Binding and activation of the receptor stimulates Janus protein kinases, which leads to the phosphorylation of several other proteins, namely STAT1 and STAT2. IFNAR1 has also been shown to interact with PRMT1 and Tyrosine kinase 2. Type I IFNs are a family of cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor and autoimmune responses In vivo.

Antigen Details

Ligand/Receptor
IFN-α/β
NCBI Gene Bank ID

References & Citations

1.) Sheehan, K. C. F. et al. (2006) JICR 26(11):804
2.) Dunn, G. P. et al. (2005) Nat. Immunol. 6(7):722
3.) Fenner, J. E. et al. (2006) Nat. Immunol. 7(1):33
4.) Lebratti, T.et al. (2021) eLife 10: e65762 Journal Link
Flow Cytometry

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.