Anti-Mouse IL-1β – Purified in vivo GOLD™ Functional Grade

Anti-Mouse IL-1β – Purified in vivo GOLD™ Functional Grade

Product No.: I-437

[product_table name="All Top" skus="I-437"]

- -
- -
Clone
B122
Target
Interleukin-1 Beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL1B, IL-1, IL1-Beta, IL1F2
Isotype
IgG
Applications
in vivo
,
IP
,
N
,
WB

- -
- -
Select Product Size

Data

- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Immunogen
E. coli -expressed, recombinant mouse IL-1β
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Additional Reported Applications For Relevant Conjugates ?
N
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Armenian Hamster Anti-Mouse IL-1β (Clone B122) recognizes Mouse IL-1β. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interleukin 1 beta (IL1 beta) is a proinflammatory cytokine belonging to IL1 family that is synthesized as a precursor. The IL1 beta precursor form is not biologically active. It is expressed mainly by stimulated macrophages and monocytes.1,2 IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. It has been recently found that IL1 beta is a major cause of severe inflammation in the mouse model of Crohns disease which makes IL1 beta an important target for this disease.3. The B122 antibody has been reported to neutralize the bioactivity of natural or recombinant IL-1β.
Antigen Distribution
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells and many other B cells, T cells, related monocytes

Antigen Details

Function
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, and glioblastoma cells
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1. Goldberg, Diamond, et. al (2017); PLoS ONE 12(5): e0177142 Article Link
1. Dinarello CA et al. (1996) Blood 87: 2095
2. Yuan et al. (1993) Cell 75: 641
3. Shin Maeda et al. (2005) Science 4: 735
in vivo Protocol
Immunoprecipitation Protocol
N
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.