Anti-Mouse IL-10 [Clone JES5-2A5] — Purified in vivo GOLD™ Functional Grade

Clone JES5-2A5 is a rat monoclonal antibody widely used in in vivo mouse studies to neutralize the activity of murine interleukin-10 (IL-10), thereby allowing researchers to examine the biological and immunoregulatory functions of IL-10 in various disease models.

The main uses in in vivo mouse studies include:

• Functional blockade of IL-10: JES5-2A5 is administered to mice (typically by intraperitoneal or intravenous injection) to specifically inhibit IL-10 signaling. This enables experiments assessing IL-10’s role in processes such as inflammation, immune regulation, and disease pathogenesis—for instance, examining susceptibility to autoimmune disorders, infectious diseases, or cancer when IL-10 activity is suppressed.
• Dissection of cytokine networks: By neutralizing IL-10, JES5-2A5 helps define the contributions of IL-10 to cytokine synthesis, T-helper cell responses, and tolerance mechanisms in vivo. For example, loss of IL-10 via JES5-2A5 treatment has been shown to produce inflammation or enhance Th1/Th17 responses in some models.
• Confirmation of IL-10-dependent mechanisms: The antibody is used in experiments to attribute phenotype changes specifically to IL-10, offering a “loss-of-function” approach that contrasts with genetic knockout models.

Additional context:

• JES5-2A5 is reported to neutralize IL-10 effectively in vivo, with exposure leading to reduced suppression of inflammatory responses usually mediated by IL-10.
• Researchers employ this clone in diverse applications including ELISA, flow cytometry, and immunohistochemistry, but only the neutralization/blocking application is relevant when discussing in vivo usage.
• Typical targets affected in studies include B cells, T cells, macrophages, and mast cells, as all are part of the IL-10 network.

In summary, JES5-2A5 is a standard tool for antibody-mediated inhibition of mouse IL-10 in vivo to elucidate IL-10’s immunoregulatory functions and its roles in inflammation, autoimmunity, infection, and tissue homeostasis.

The correct storage temperature for the sterile packaged clone JES5-2A5 is 2°C to 8°C.

This temperature range applies to both short-term and standard storage conditions for the undiluted antibody solution in sterile packaging. For extended long-term storage (longer than one month), it is recommended to aliquot the antibody and store at -80°C to preserve its functionality, avoiding repeated freeze-thaw cycles. Handling under aseptic conditions is important to maintain sterility.

• Short-term/standard storage: 2°C–8°C (refrigerator; do not freeze).
• Long-term storage (over one month): Aliquot and store at -80°C.

The product should always be protected from repeated freezing and thawing, which can damage the antibody.

Other commonly used antibodies or proteins with JES5-2A5 in the literature include JES5-16E3 and SXC-1, which are often paired for the detection or quantification of mouse IL-10 in ELISA, ELISPOT, and related assays.

• JES5-16E3: Frequently used as a detecting antibody alongside JES5-2A5 as the capture antibody in sandwich ELISA and ELISPOT formats. The pairing of JES5-2A5 with JES5-16E3 is well-documented for sensitive and specific detection of mouse IL-10.
• SXC-1: Another biotinylated antibody often used with JES5-2A5, particularly in BD Biosciences ELISA protocols, where purified JES5-2A5 serves as capture and SXC-1 as detection.
• Rat IgG1 Isotype Control: Used to control for non-specific binding in assays with JES5-2A5, specifically the clone KLH/G1-2-2.
• Common Proteins/Targets in Studies: While JES5-2A5 is specific to mouse IL-10, studies employing this antibody often include panels of cytokine antibodies (e.g., anti-IL-6, anti-TNF-?, anti-IFN-?) or cell markers (e.g., CD4, CD8, CD19) to characterize immune responses, though individual antibody clones may vary by experiment.

In ELISA and ELISPOT, the standard combination is JES5-2A5 for capture and JES5-16E3 for detection (or vice versa if conjugated forms are swapped). In flow cytometry, immunohistochemistry, and western blot studies using JES5-2A5, researchers may also co-stain or probe for other cytokines or immune cell lineage markers to analyze broader immune features.

These combinations are reported in manufacturer protocols and the scientific literature, particularly in studies focused on mouse models of inflammation, autoimmunity, or infection.

Clone JES5-2A5 is a widely used rat monoclonal antibody that specifically targets mouse interleukin-10 (IL-10) and is primarily applied to neutralize the activity of murine IL-10 in various immunological studies.

Key findings from citations of clone JES5-2A5 in scientific literature include:

• Biological Role of IL-10: JES5-2A5 has been instrumental in elucidating the anti-inflammatory properties of IL-10 in mice, including its roles in inhibiting pro-inflammatory cytokine synthesis, promoting B cell survival, and regulating macrophage and T cell-mediated immune responses. The antibody's ability to neutralize IL-10 helped demonstrate how IL-10 prevents excessive immune activation and autoimmunity.
• Experimental Applications: The antibody is routinely used in neutralization assays, Western blotting, flow cytometry, ELISA, immunohistochemistry, and blocking/inhibition studies to assess IL-10 function in vivo and in vitro.
• Immune Regulation: Through JES5-2A5-mediated IL-10 blockade, studies have shown IL-10 as essential for maintaining immune tolerance to self-antigens, allergens, and transplantation antigens, as well as limiting tissue damage during infection by damping inflammatory responses.
• Disease Models: Knockout or neutralization studies using JES5-2A5 revealed that the lack of IL-10 leads to inflammation, pain, and vascular dysfunction due to unrestrained immune responses, underlining IL-10's protective effect, especially in models of intestinal inflammation and cardiovascular pathologies.
• Mechanistic Insights: JES5-2A5 helped detail the molecular signaling pathways controlled by IL-10, such as inhibition of NF-?B and modulation of the JAK-STAT pathway.
• Macrophage Polarization: Recent work (e.g., in Nature 2021) has used JES5-2A5 in experiments showing that certain signals (like B cell-derived GABA) drive the development of anti-inflammatory, IL-10-producing macrophages, thereby limiting cytotoxic T cell activity and restraining inflammation.
• Technical and Experimental Versatility: JES5-2A5 is validated across experimental methods and is frequently cited in immunological literature for its reliability in neutralizing endogenous IL-10, identifying IL-10-expressing cells, and dissecting IL-10-mediated pathways in mouse models.

In summary, JES5-2A5 citations consistently highlight its central role in defining the immunosuppressive, regulatory, and tissue-protective functions of IL-10 in mouse immunology, both under physiological and disease conditions.