Anti-Mouse IL-10 – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.I-1152
Clone
JES5-2A5
Protein
IL-10
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CSIF, IL10A, MGC126450, MGC126451, TGIF
Isotype
Rat
IgG1 κ
Applications
CyTOF®
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-1152-0.5 mg
0.5 mg
100.00
In stock
Max:
Min: 1
Step: 1
I-1152-1.0 mg
1.0 mg
175.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Sample Reactivity Species
Host Species
Rat
Immunogen
Purified Recombinant Mouse IL-10
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Other Applications Reported In Literature ?
CyTOF®
Additional Reported Applications For Relevant Conjugates ?
N
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone JES5-2A5 recognizes mouse IL-10.
Antigen Distribution
Sample Antigen Distribution
Background
IL-10 is a homodimeric, anti-inflammatory cytokine of 17-21 kD with various, pleiotropic, effects in immunoregulation and inflammation. It increases antibody production, in addition to enhancing B cell survival and proliferation. IL-10 inhibits both the synthesis of pro-inflammatory cytokines and the actions of NK cells during the immune response to viral infection. Moreover, IL-10 is involved in peripheral T cell tolerance to allergens, autoantigens, transplantation antigens and tumor antigens. IL-10 can also block NF-B activity, and is involved in the regulation of the JAK-STAT signaling pathway. In mice, lack of IL-10 has been shown to cause inflammation and pain via COX activation resulting in vascular endothelial and cardiac dysfunctions. Additionally, IL-10 is linked to myokines, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis in the immune system. Exercise is known to increase circulating levels of IL-10. Hence, it is thought that physical exercise promotes an environment of anti-inflammatory cytokines. Furthermore, knockout studies of IL-10 suggest this cytokine is crucial for counteracting the hyperactive immune response in the intestinal tract. It has been reported that treatment with recombinant IL-10 producing bacteria has been beneficial in patients with Crohn's disease.

Antigen Details

Protein
Function
Sample Function
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Akdis, CA. et al. (2009) Mol. Immunol. 46: 622
2. Medzhitov, R. et al. (2017) Science. 356(6337):513-519.
General Western Blot Protocol
No results found. Clear filters and try again?

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.