Anti-Mouse IL-17D (Clone 312724)
Anti-Mouse IL-17D (Clone 312724)
Product No.: I-880
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Clone 312724 Target IL-17D Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-17D Isotype IgG2a Applications CyTOF® , FC , WB |
Data
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Immunogen Purified Recombinant Mouse IL-17D (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830965 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-17D. A suggested method would be to stain cells expressing IL-17D with 2.5 µg/106 cells in a total staining volume of 200 µl followed by PN:M1189. Western Blotting: To detect Mouse IL-17D this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-17D is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-332. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Mouse Interleukin 17D (IL-17D) (Clone 312724) recognizes an epitope on Mouse IL-17D. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical ProtocolsCyTOF®   |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
