Anti-Mouse IL-21R (Clone 4A9) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse IL-21R (Clone 4A9) – Purified in vivo PLATINUM™ Functional Grade

Product No.: I-479

[product_table name="All Top" skus="F1196"]

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Clone
4A9
Target
IL-21R
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
Interleukin-21 Receptor Subunit, CD360
Isotype
Rat IgG2a κ
Applications
FA
,
FC
,
in vivo
,
IP

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Rat YB2/0 hybridoma transfectant cell line expressing truncated IL-21R
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
liquid
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Additional Applications Reported In Literature ?
FA,
IP,
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 4A9 activity is directed against murine Interleukin-21 Receptor (CD360).
Background
Interleukin-21 receptor (IL-21R) is a single pass type I membrane protein and a member of the type I cytokine receptor family. It is the receptor for Interleukin-21 (IL-21) and upon binding forms a heterodimer with the common gamma subunit (CD132; IL-2RG). IL-21R and IL-21 are important for the proliferation and differentiation of B cells, T cells, and natural killer cells. They function in survival signalling for antiviral CD8+ T cells during chronic infections and induce growth and differentiation of mature B cells in Ig-secreting plasma cells. Additionally, they prevent T cell exhaustion and viral persistence by limiting virus driven Treg cell proliferation1. IL-21 and IL21-R function as early as the pro-B cell stage, with receptor-mediated signalling increasing as cells mature2. When IL-21R binds IL-21, the JAK/STAT transduction pathway is activated.

4A9 was generated by immunizing Lewis rats with IL-21R-transfected YB2/0 and subsequent fusion of immunized spleen cells to SP2/03. FACS analysis and immunoprecipitation studies show that 4A9 selectively binds to IL-21R. Additionally, 4A9 stains the majority of spleen cells from wild type C57BL/6 mice but not IL-21R deficient mice. IL-21 blocks the binding of 4A9 to IL-21R-transfected YB2/0 cells.
Antigen Distribution
IL-21R is expressed on most B cells as well as some T cells and NK cells.

Antigen Details

Ligand/Receptor
IL-21
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Schmitz I, Schneider C, Fröhlich A, et al. PLoS Pathog. 9(5):e1003362. 2013.
2. Simard N, Konforte D, Tran AH, et al. J Immunol. 186(9):5244-5253. 2011.
3. Jin H, Carrio R, Yu A, et al. J Immunol. 173(1):657-665. 2004.
4. DeKoter RP, Geadah M, Khoosal S, et al. J Immunol. 185(12):7374-7384. 2010.
FA
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.