Anti-Mouse IL-4 [Clone 11B11] — Alexa Fluor® 647

Partially Purified Native Mouse IL-4

This Alexa Fluor® 647 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

This Alexa Fluor® 647 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Red Laser (633 nm) Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Clone 11B11 recognizes an epitope on mouse IL-4.

IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils^1,2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells^3,4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes⁵. In addition, IL-4 is implicated in normal wound healing^6,7 and airway inflammation in patients with asthma⁸.

IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils.

1. Yoshimoto T & Paul WE. (1994) J Exp Med. 179(4):1285–95
2. Kipnis J., at al. (2012) J Immunol. 189(9):4213-4219
3. Groth B., et al. (1992) J Exp Med. 176:1091–8
4. Murphy KM., et al. (1992) Proc Natl Acad Sci USA. 89:6065–9
5. Paul WE., et al. (1999) Annual Rev Immunol. 17:701-38
6. Maquart FX., et al. (2000) Lab Invest. 80(8):1337-43
7. Godeau, G., et al. (2000) Lab Invest. 80,1337–1343
8. Steinke JW & Borish L. (2001) Respir Res. 2(2):66-70