Anti-Mouse IL-4 (Clone 11B11) – Purified in vivo PLATINUM™ Functional Grade
Pricing & Details
Purified Recombinant Mouse IL-4
≥ 5.0 mg/ml
≤ 0.5 EU/mg as determined by the LAL method
≥98% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISAThis antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 μg/ml. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone 11B11 recognizes an epitope on mouse IL-4.
IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils.
IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils1,2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells3,4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes5. In addition, IL-4 is implicated in normal wound healing6,7 and airway inflammation in patients with asthma8.
NCBI Gene Bank ID
References & Citations
1. Yoshimoto T & Paul WE. (1994) J Exp Med. 179(4):1285–95
2. Kipnis J., at al. (2012) J Immunol. 189(9):4213-4219
3. Groth B., et al. (1992) J Exp Med. 176:1091–8
4. Murphy KM., et al. (1992) Proc Natl Acad Sci USA. 89:6065–9
5. Paul WE., et al. (1999) Annual Rev Immunol. 17:701-38
6. Maquart FX., et al. (2000) Lab Invest. 80(8):1337-43
7. Godeau, G., et al. (2000) Lab Invest. 80,1337–1343
8. Steinke JW & Borish L. (2001) Respir Res. 2(2):66-70