Anti-Mouse IL-4 (Clone 11B11) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.I-2070
Clone
11B11
Protein
IL-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, IaIF, HCGF, MCGF2, MFF, TCGF-2
Isotype
Rat
IgG1 κ
Applications
CyTOF®
,
ELISA Cap
,
ELISPOT
,
FA
,
ICC
,
IHC
,
IP
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-2070-1 mg
1 mg
$225.00
$175.50
In stock
Max:
Min: 1
Step: 1
I-2070-5 mg
5 mg
$445.00
$347.10
In stock
Max:
Min: 1
Step: 1
I-2070-25 mg
25 mg
$1,285.00
$1,002.30
In stock
Max:
Min: 1
Step: 1
I-2070-50 mg
50 mg
$2,045.00
$1,595.10
In stock
Max:
Min: 1
Step: 1
I-2070-100 mg
100 mg
$2,800.00
$2,184.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse IL-4
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISAThis antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 μg/ml. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
N
IHC
ICC
IP
CyTOF®
FA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 11B11 recognizes an epitope on mouse IL-4.
Antigen Distribution
IL-4 is secreted by Th2 cells, NKT cells, mast cells, eosinophils, and basophils.
Background
IL-4 antibody, 11B11, recognizes mouse IL-4, also known as B-cell differentiation factor (BCDF) and B-cell stimulatory factor (BSF1). IL-4 is a pleiotropic 20 kDa cytokine secreted by T helper 2 cells (Th2) cells, natural killer T (NKT) cells, mast cells, eosinophils, and basophils1,2. IL-4 signaling through the IL-4 receptor alpha chain (IL-4Ra) induces the differentiation of naive T cells to Th2 cells3,4 and stimulates B cell differentiation, proliferation, and class switching to IgG1 and IgE isotypes5. In addition, IL-4 is implicated in normal wound healing6,7 and airway inflammation in patients with asthma8.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Yoshimoto T & Paul WE. (1994) J Exp Med. 179(4):1285–95
2. Kipnis J., at al. (2012) J Immunol. 189(9):4213-4219
3. Groth B., et al. (1992) J Exp Med. 176:1091–8
4. Murphy KM., et al. (1992) Proc Natl Acad Sci USA. 89:6065–9
5. Paul WE., et al. (1999) Annual Rev Immunol. 17:701-38
6. Maquart FX., et al. (2000) Lab Invest. 80(8):1337-43
7. Godeau, G., et al. (2000) Lab Invest. 80,1337–1343
8. Steinke JW & Borish L. (2001) Respir Res. 2(2):66-70
Elisa Sandwich Protocol
IHC
Immunoprecipitation Protocol