Anti-Mouse IL-7 Rα – Biotin

Anti-Mouse IL-7 Rα – Biotin

Product No.: I-795

[product_table name="All Top" skus="I-795"]

- -
- -
Target
IL-7 Rα
Product Type
Polyclonal Antibody
Alternate Names
Interleukin-7 Receptor Alpha , IL7R, CD127, CDW127
Applications
FC
,
WB

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse IL-7 Rα (Accession # P16872)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-7 Rα. A suggested method would be to stain cells expressing IL-7 Rα with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.
Western Blotting: To detect Mouse IL-7 Rα this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-7 Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-581.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Interleukin 7 Receptor Alpha (IL-7 Rα) recognizes Mouse IL-7 Rα. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.
Antigen Distribution
IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Fry, TJ. et al. (2005) J Immunol. 174: 6571
2. Murre, C. et al. (2002) Embo J. 21: 103
Flow Cytometry
General Western Blot Protocol

Certificate of Analysis

- -
- -
Prod No.
Description
I-795
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.