Anti-Mouse Lefty-1

Product No.: L220


Clone 146903 Target Lefty-1 Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2a Applications ELISA Indirect , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse Lefty-1 Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831150 Applications and Recommended Usage? Quality Tested by Leinco Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse Lefty-1 is approximately 3 ng/well. Western Blotting: To detect Mouse Lefty-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Lefty-1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Rat Anti-Mouse Lefty-1 (Clone 146903) recognizes an epitope on Mouse Lefty-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed Lefty-1 NCBI Gene Bank ID 10637 UniProt.org More Information on Uniprot.org References & Citations View product citations for antibody L220 on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

NS0-Derived Recombinant Mouse Lefty-1

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831150

Applications and Recommended Usage?
Quality Tested by Leinco

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:R380. The detection sensitivity of this indirect ELISA for Mouse Lefty-1 is approximately 3 ng/well.
Western Blotting: To detect Mouse Lefty-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Lefty-1 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse Lefty-1 (Clone 146903) recognizes an epitope on Mouse Lefty-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

Lefty-1

NCBI Gene Bank ID

10637

UniProt.org

More Information on Uniprot.org