Anti-Mouse LIX – Biotin

Pricing & Details

Product No.L140
Protein
LIX
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
CXCL5, AMCF-II, ENA-78, GCP-2, Scyb5, Scyb6, Lipopolysaccharide-Induced CXC Chemokine (LIX)
Applications
ELISA Det
,
WB
Prod No.
Size
Price
Avail.
Qty
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L140-50 µg
50 µg
$525.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse CXCL5 (Accession # P50228)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CXCL5 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L123.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN: C1414 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse CXCL5.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse CXCL5 recognizes Mouse CXCL5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
CXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family1 that has a role in the development of cardiovascular diseases.2 CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.1 It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.3 CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.4

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Rajashekhar, G. et al. (2007) Physiol Genomics 31: 104
2. Mahsa, M. et al. (2008) Molec Diagnosis & Therapy 12: 391
3. Wågsäter, D. et al. (2007) International J Oncol. 31: 97
4. Nagelkerke, F. et al. (2008) Clinical Cancer Research 14: 2276
Indirect Elisa Protocol
General Western Blot Protocol
Prod No.
Description
L140
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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