Anti-Mouse LPAM-1 – APC

Anti-Mouse LPAM-1 – APC

Product No.: L295

[product_table name="All Top" skus="L295"]

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Clone
DATK32
Target
Integrin α4β7
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
α4β7 Integrin, CD49d/β7
Isotype
IgG2a κ
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
TK1 Cells
Product Concentration
0.2 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Storage and Handling
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (650 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this DATK32 antibody for staining cells in flow cytometry is ≤0.25 µg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
B This antibody has been reported to block α4β7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin for In vitro and In vivo studies.
IHC FF
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone DATK32 recognizes an epitope specific to the mouse LPAM-1 heterodimer.
Antigen Distribution
LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow progenitor cells.
Background
LPAM-1 antibody, clone DATK32, recognizes mouse lymphocyte Peyer’s patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7 (α4β7). LPAM-1 is a heterodimer of the 154 kDa integrin α4 (CD49d) and 130 kDa integrin β7 chains and is a member of the Ig superfamily. LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow hematopoietic stem cells1,2. LPAM-1 mediates adhesion to endothelial cells, promoting leukocyte transmigration across high endothelial venules (HEVs) during the inflammatory response. LPAM-1 binds to several ligands, including vascular adhesion molecule-1 (VCAM-1/CD106), mucosal addressin cell adhesion molecule-1 (MAdCAM-1), and fibronectin. MAdCAM-1 is expressed exclusively on endothelial cells of the gut and gut-associated lymphoid tissues, such as Peyer’s patches (PPs), and binding of LPAM-1 to MAdCAM-1 contributes to lymphocyte homing to mucosal tissues3,4. Vedolizumab, an anti-LPAM-1 monoclonal antibody, is approved to treat inflammatory bowel disease and effectively induces and maintains remission in Crohn's disease and ulcerative colitis5-8.

Antigen Details

Ligand/Receptor
VCAM-1 (CD106), MAdCAM-1, fibronectin
Function
Lymphocyte adhesion
NCBI Gene Bank ID
Research Area
Cell Biology
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Immunology
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Neuroscience

References & Citations

1. Andrew DP, et al. (1996) Eur J Immunol. 26(4):897-905
2. Murakami JL, et al. (2016) Stem Cells Dev. 25(1):18-26
3. Hu MC, et al. (1992) Proc Natl Acad Sci USA. 89(17):8254-8
4. Hamann A, et al. (1994) J Immunol. 152(7):3282-93
5. Sandborn WJ, et al. (2013) N Engl J Med. 369:711–721
6. Feagan BG, et al. (2013) N Engl J Med. 369:699–710
7. Kopylov U, et al. (2019) Dig Liver Dis. 51:68–74
8. Danese S, et al. (2019) Gastroenterology.157:1007–1018 e1007
Flow Cytometry

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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