Anti-Mouse LPAM-1 – Purified in vivo GOLD™ Functional Grade

Anti-Mouse LPAM-1 – Purified in vivo GOLD™ Functional Grade

Product No.: L307

[product_table name="All Top" skus="L307"]

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Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
α4β7 Integrin, CD49d/β7, LPAM-1, ITGA4, ITGB7
IgG2a κ
in vivo

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Antibody Details

Product Details

Reactive Species
Host Species
TK1 cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this DATK32 antibody for staining cells in flow cytometry is ≤1.0 µg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
B This antibody has been reported to block α4β7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin for In vitro and In vivo studies.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Clone DATK32 recognizes an epitope specific to the mouse LPAM-1 heterodimer.
LPAM-1 antibody, clone DATK32, recognizes mouse lymphocyte Peyer’s patch adhesion molecule (LPAM-1), also known as integrin alpha 4 beta 7 (α4β7). LPAM-1 is a heterodimer of the 154 kDa integrin α4 (CD49d) and 130 kDa integrin β7 chains and is a member of the Ig superfamily. LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow hematopoietic stem cells1,2. LPAM-1 mediates adhesion to endothelial cells, promoting leukocyte transmigration across high endothelial venules (HEVs) during the inflammatory response. LPAM-1 binds to several ligands, including vascular adhesion molecule-1 (VCAM-1/CD106), mucosal addressin cell adhesion molecule-1 (MAdCAM-1), and fibronectin. MAdCAM-1 is expressed exclusively on endothelial cells of the gut and gut-associated lymphoid tissues, such as Peyer’s patches (PPs), and binding of LPAM-1 to MAdCAM-1 contributes to lymphocyte homing to mucosal tissues3,4. Vedolizumab, an anti-LPAM-1 monoclonal antibody, is approved to treat inflammatory bowel disease and effectively induces and maintains remission in Crohn's disease and ulcerative colitis5-8.
Antigen Distribution
LPAM-1 is expressed on most peripheral lymphocytes, subsets of thymocytes, and bone marrow progenitor cells.

Antigen Details

VCAM-1 (CD106), MAdCAM-1, fibronectin
Lymphocyte adhesion
NCBI Gene Bank ID
Research Area
Cell Biology

References & Citations

1. Andrew DP, et al. (1996) Eur J Immunol. 26(4):897-905
2. Murakami JL, et al. (2016) Stem Cells Dev. 25(1):18-26
3. Hu MC, et al. (1992) Proc Natl Acad Sci USA. 89(17):8254-8
4. Hamann A, et al. (1994) J Immunol. 152(7):3282-93
5. Sandborn WJ, et al. (2013) N Engl J Med. 369:711–721
6. Feagan BG, et al. (2013) N Engl J Med. 369:699–710
7. Kopylov U, et al. (2019) Dig Liver Dis. 51:68–74
8. Danese S, et al. (2019) Gastroenterology.157:1007–1018 e1007
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.