Anti-Mouse Ly-6C-BX037 (HK1.4); Alexa Fluor™ 750-RX037
Anti-Mouse Ly-6C-BX037 (HK1.4); Alexa Fluor™ 750-RX037
Product No.: L405
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Clone HK1.4 Target Ly-6C Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Lymphocyte antigen 6 complex, locus C Isotype IgG2c Applications IHC FF , PhenoCycler® |
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CODEX® DetailsBarcode BX037 Reporter Alexa Fluor™ 750-RX037 Fluorescent Dye Alexa Fluor™ 750 is in the infrared fluorescent channel with an excitation max of 749 nm and an emission max of 775 nm. Tissue Screened MCA Sarcoma (Tumor) Tissue Preparation FF (Fresh Frozen) Antibody and Reporter DetailsReactivity Species Mouse Host Species Rat Immunogen L3 cloned CTL cells Formulation This PhenoCycler-Fusion (CODEX)® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0) Product Preparation Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions. State of Matter CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized Storage and Handling This PhenoCycler-Fusion (CODEX)® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year. Applications and Recommended Usage? Quality Tested by Leinco CODEX®; IHC FF - The suggested dilution for staining tissue in immunohistochemistry - Fresh Frozen (FF) is 1 μl of Anti-Mouse Ly-6C (BX037) in a final volume of 200 μl of CODEX® staining buffer. RRID AB_2893100 Country of Origin USA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Ly-6C (Clone HK1.4) recognizes an epitope on Mouse Ly-6C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen Distribution The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5
Background Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells. Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation.
PhenoCycler-Fusion (CODEX)® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a CODEX® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes conjugated to antibodies. The use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time. Antigen DetailsProtein PubMed NCBI Gene Bank ID References & CitationsHavran, W. L. et al. (1988) J. Immunol. 140(4):1034 Technical Protocols PhenoCycler® |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
