Anti-Mouse Ly-6C-BX037 (HK1.4); Alexa Fluor™ 750-RX037

Pricing & Details

Product No.L405
Clone
HK1.4
Protein
Ly-6C
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat
IgG2c
Applications
CODEX®
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
L405-25 Tests
25 tests
$400.00
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Min: 1
Step: 1

CODEX® Details

Barcode
BX037
Reporter
Alexa Fluor™ 750-RX037
Fluorescent Dye
Alexa Fluor™ 750 is in the infrared fluorescent channel with an excitation max of 749 nm and an emission max of 775 nm.
Tissue Screened
MCA Sarcoma (Tumor)
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
This CODEX® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Product Preparation
Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.
State of Matter
CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized
Storage and Handling
This CODEX® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX®; IHC FF - The suggested dilution for staining tissue in immunohistochemistry - Fresh Frozen (FF) is 1 μl of Anti-Mouse Ly-6C (BX037) in a final volume of 200 μl of CODEX® staining buffer.
RRID
AB_2893100
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Ly-6C (Clone HK1.4) recognizes an epitope on Mouse Ly-6C. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The antigen is found on some monocyte/macrophage populations, endothelial cells, thymocytes, NK-cells, T-cell subsets and 40% of bone marrow cells from all mouse strains tested. Clone HK1.4 does not block the binding of clone RB6-8C5
Background
Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells. Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation.

CODEX® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a CODEX® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes conjugated to antibodies. The use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

Havran, W. L. et al. (1988) J. Immunol. 140(4):1034
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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