Anti-Mouse Ly-6G/Ly-6C (Gr-1) [Clone RB6-8C5] — Alexa Fluor® 488

Purified Recombinant Mouse Granulocytes (>98%)

This Alexa Fluor® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

This Alexa Fluor® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Blue Laser (488 nm) Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Rat Anti-Mouse Granulocytes (Clone RB6-8C5) recognizes an epitope on Mouse Granulocytes. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Gr-1 (Ly-6G/Ly-6C) is a 21–25 kDa glycosylphosphatidylinositol (GPI)-linked cell surface protein expressed on myeloid lineage cells, specifically granulocytes and macrophages. In the bone marrow, Gr-1 expression directly correlates with granulocyte differentiation and maturation, while also showing transient expression on monocyte precursors. Additionally, immature Gr-1⁺ myeloid cells play a critical role in mediating antitumor immune responses.

The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.^1,2

1. Fleming, T. J. et al. (1993) J. of Immunol. 151(5):2399
2. Hestdal, K. et al. (1991) J. of Immunol. 147(1):22
3. Brummer, E. et al. (1984) J. Leuko. Bio. 36:505