Anti-Mouse Ly-6G/Ly-6C (Gr-1) – Purified In vivo GOLD™ Functional Grade

Pricing & Details

  
Clone
RB6-8C5
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Gr-1
Isotype
Rat
IgG2b
Prod No.
Size
Price
Avail.
Qty
Add to cart
G100-1.0 mg
1.0 mg
$95.00
In stock
G100-5.0 mg
5.0 mg
$298.00
In stock
G100-25 mg
25 mg
$775.00
In stock
G100-50 mg
50 mg
$1,275.00
In stock
G100-100 mg
100 mg
$1,998.00
In stock
  

Antibody Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Product Concentration
0.5 mg/ml
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Other Applications Reported In Literature ?
CyTOF®
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Rat Anti-Mouse GR-1, as known as anti-mouse Ly-6G/Ly-6C (Clone RB6-8C5) has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2
Background
Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

Antigen Details

Protein Family
Ly-6G/Ly-6C
NCBI Gene Bank ID

References & Citations

1.) Yokoyama, Hitoshi et al. J Am Soc Nephrol. 2003 Oct;14(10):2503-15. PubMed
2.) Fleming, T. J. et al. (1993) J. of Immunol. 151(5):2399
3.) Hestdal, K. et al. (1991) J. of Immunol. 147(1):22
4.) Brummer, E. et al. (1984) J. Leuko. Bio. 36:505
Immunohistochemistry
Immunoprecipitation Protocol
General Western Blot Protocol
  

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.