Anti-Mouse Ly-6G/Ly-6C (Gr-1) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse Ly-6G/Ly-6C (Gr-1) – Purified in vivo GOLD™ Functional Grade

Product No.: G100

[product_table name="All Top" skus="G100"]

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Clone
RB6-8C5
Target
Ly-6G/Ly-6C
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Gr-1
Isotype
IgG2b
Applications
CyTOF®
,
Depletion
,
IHC FF
,
in vivo
,
IP
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 μg per 106 cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
CyTOF®
Depletion
Clone RB6-8C5 is suitable for In vivo depletion. However, it has been reported that clone RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs).
IHC (Frozen)
IHC (Paraffin)
IP
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse GR-1, as known as anti-mouse Ly-6G/Ly-6C (Clone RB6-8C5) has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2
Background
Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

Antigen Details

NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1.) Yokoyama, Hitoshi et al. J Am Soc Nephrol. 2003 Oct;14(10):2503-15. PubMed
2.) Fleming, T. J. et al. (1993) J. of Immunol. 151(5):2399
3.) Hestdal, K. et al. (1991) J. of Immunol. 147(1):22
4.) Brummer, E. et al. (1984) J. Leuko. Bio. 36:505
CyTOF®
Depletion
IHC FF
in vivo Protocol
Immunoprecipitation Protocol
General Western Blot Protocol
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.