Anti-Mouse Ly-6G/Ly-6C (Gr-1) – Purified in vivo PLATINUM™ Functional Grade

Home/Bulk In vivo Functional Grade Antibodies/Platinum Purified Bulk Antibodies/Anti-Mouse Ly-6G/Ly-6C (Gr-1) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Prod. No.G153
Clone
RB6-8C5
Product Type
Monoclonal Antibody
Alternate Names
Ly-6G/LY-6C, Gr-1, Myeloid
Isotype
Rat
IgG2b
Workshops
Size
Price
Add to cart
1.0 mg
198.00
Max:
Min: 1
Step: 1
5.0 mg
398.00
Max:
Min: 1
Step: 1
25 mg
1,150.00
Max:
Min: 1
Step: 1
50 mg
1,825.00
Max:
Min: 1
Step: 1
100 mg
2,500.00
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 μg per 106 cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
CyTOF®
Depletion
Clone RB6-8C5 is suitable for In vivo depletion. However, it has been reported that clone RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs).
IHC (Frozen)
IHC (Paraffin)
IP
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Granulocytes (Clone RB6-8C5) recognizes Mouse Granulocytes . This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody was also test by PCR and IMPACT1 certified (For more information contact your Sales Rep).
Antigen Distribution
The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1,2
Background
Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

Antigen Details

Protein
PubMed
Research Area
Immunology
.
Innate Immunity

References & Citations

1.) Yokoyama, Hitoshi et al. J Am Soc Nephrol. 2003 Oct;14(10):2503-15. PubMed
2.) Fleming, T. J. et al. (1993) J. of Immunol. 151(5):2399
3.) Hestdal, K. et al. (1991) J. of Immunol. 147(1):22
4.) Brummer, E. et al. (1984) J. Leuko. Bio. 36:505
Flow Cytometry
IHC
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available