Anti-Mouse MHC Class I (H-2Db) (Clone 28-14-8S) – PE
Anti-Mouse MHC Class I (H-2Db) (Clone 28-14-8S) – PE
Product No.: H225
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Clone 28-14-8S Target MHC Class I (H-2Db) Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Isotype Mouse IgG2a k Applications B , FA , FC , IHC , IP |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Immunogen C3H.SW (H-2b) spleen cells Product Concentration 0.2 mg/ml Formulation This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. State of Matter Liquid Storage and Handling This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Excitation Laser Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm) Additional Applications Reported In Literature ? B, FA, FC, IHC, IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 28-14-8S activity is directed against the α3 domain of mouse MHC Class I H-2Db. Background H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of
antigens divided into class I and II proteins that function in immune response1. H-2Db is a class I MHC cell surface protein. MHC class I molecules consist of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin)2,3. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins which are then displayed on the cell surface1 to CD8 T cells via a process known as cross-presentation4. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection5. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell1. 28-14-8S was generated by immunizing BALB/c C3H/HeJ (H-2k) mice with C3H.SW (H-2b) spleen cells6. Spleen cells were then fused with SP2/0 AG14 to create a hybridoma line. 28-14-8S does not react with Kd or Dd antigens. 28-14-8S binds to native molecules and free heavy chains7. Antigen Distribution H-2Db is expressed on the cell surface of nucleated cells from mice of the H-2Db haplotype.
Ligand/Receptor CD3/TCR, CD8 NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity References & Citations1. Yoshida R. Adv Immunol. 124:207-247. 2014. 2. Mitaksov V, Fremont DH. J Biol Chem. 281(15):10618-10625. 2006. 3. Wieczorek M, Abualrous ET, Sticht J, et al. Front Immunol. 8:292. 2017. 4. Cruz FM, Colbert JD, Merino E, et al. Annu Rev Immunol. 35:149-176. 2017. 5. Peng S, Mattox A, Best SR, et al. Cancer Immunol Immunother. 65(3):261-271. 2016. 6. Ozato K, Hansen TH, Sachs DH. J Immunol. 125(6):2473-2477. 1980. 7. Marguet D, Spiliotis ET, Pentcheva T, et al. Immunity. 11(2):231-240. 1999. 8. Bashuda H, Seino K, Ra C, et al. Transplantation. 63(1):113-118. 1997. 9. Frey BF, Jiang J, Sui Y, et al. J Immunol. 200(5):1853-1864. 2018. 10. Kafrouni MI, Brown GR, Thiele DL. J Immunol. 167(3):1566-1574. 2001. 11. Hutton C, Heider F, Blanco-Gomez A, et al. Cancer Cell. 39(9):1227-1244. 2021. Technical ProtocolsCertificate of Analysis |
Formats Available
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