Anti-Mouse MHC Class I (H-2Db) (Clone 28-14-8S) – PE

Anti-Mouse MHC Class I (H-2Db) (Clone 28-14-8S) – PE

Product No.: H225

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Clone
28-14-8S
Target
MHC Class I (H-2Db)
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Isotype
Mouse IgG2a k
Applications
B
,
FA
,
FC
,
IHC
,
IP

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Immunogen
C3H.SW (H-2b) spleen cells
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Applications Reported In Literature ?
B,
FA,
FC,
IHC,
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
28-14-8S activity is directed against the α3 domain of mouse MHC Class I H-2Db.
Background
H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. H-2Db is a class I MHC cell surface protein. MHC class I molecules consist of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin)2,3.

MHC class I molecules bind peptides generated by the degradation of cytosolic proteins which are then displayed on the cell surface1 to CD8 T cells via a process known as cross-presentation4. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. For example, H-2Db plays a role in human papillomavirus infection5. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell1.

28-14-8S was generated by immunizing BALB/c C3H/HeJ (H-2k) mice with C3H.SW (H-2b) spleen cells6. Spleen cells were then fused with SP2/0 AG14 to create a hybridoma line. 28-14-8S does not react with Kd or Dd antigens. 28-14-8S binds to native molecules and free heavy chains7.

Antigen Distribution
H-2Db is expressed on the cell surface of nucleated cells from mice of the H-2Db haplotype.
Ligand/Receptor
CD3/TCR, CD8
NCBI Gene Bank ID
UniProt.org
Research Area
Immunology
.
Innate Immunity

References & Citations

1. Yoshida R. Adv Immunol. 124:207-247. 2014.
2. Mitaksov V, Fremont DH. J Biol Chem. 281(15):10618-10625. 2006.
3. Wieczorek M, Abualrous ET, Sticht J, et al. Front Immunol. 8:292. 2017.
4. Cruz FM, Colbert JD, Merino E, et al. Annu Rev Immunol. 35:149-176. 2017.
5. Peng S, Mattox A, Best SR, et al. Cancer Immunol Immunother. 65(3):261-271. 2016.
6. Ozato K, Hansen TH, Sachs DH. J Immunol. 125(6):2473-2477. 1980.
7. Marguet D, Spiliotis ET, Pentcheva T, et al. Immunity. 11(2):231-240. 1999.
8. Bashuda H, Seino K, Ra C, et al. Transplantation. 63(1):113-118. 1997.
9. Frey BF, Jiang J, Sui Y, et al. J Immunol. 200(5):1853-1864. 2018.
10. Kafrouni MI, Brown GR, Thiele DL. J Immunol. 167(3):1566-1574. 2001.
11. Hutton C, Heider F, Blanco-Gomez A, et al. Cancer Cell. 39(9):1227-1244. 2021.
B
FA
Flow Cytometry
IHC
Immunoprecipitation Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.