Anti-Mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As,I-Ag7) [Clone 10-3.6.2] — Purified in vivo GOLD™ Functional Grade

C3H mouse spleen cells

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 8° C Wet Ice

10-3.6.2 activity is directed against the mouse major histocompatibility complex I-Ak, I-Ar, I-Af, I-As, and I-Ag7 haplotypes but does not react with I-A of the b, d, p and q haplotypes.

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response¹. Class II molecules, also known as Ia antigens, regulate recognition of foreign antigens on the surfaces of antigen presenting cells and play a major role in the mixed lymphocyte response². Mice have two class II isotypes, I-A and I-E, each of which is a glycoprotein composed of an ⍺ and β subunit. The N-terminal α1 and β1 domains of the MHC class II isotype form the antigen- binding groove, which binds 13-25 aa peptides derived from exogenous antigens³.

On APCs, MHC class II molecules play a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling⁴. Additionally, MHC class II expressed on activated T cells are capable of antigen presentation⁵ and can transduce signals into T cells, enhancing T cell proliferation and activity⁶.

10-3.6.2 was generated by immunizing CWB mice with spleen cells from C3H mice⁷. The resulting spleen cells were fused with NS-1 myeloma cells. Hybridomas were screened by plate binding assay using spleen cells from H-2 and Ig congenic mouse strains as targets. A series of recombinant strains of mice were then used to map the antibody to regions of the MHC complex.

MHC class II molecules are constitutively expressed on professional antigen-presenting cells (APCs), including macrophages, monocytes, dendritic cells (DCs), and B cells, and are induced on T cells upon activation

CD3/TCR, CD4

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9 Liu Z, Aune TM. Genes Immun. 7(5):352-358. 2006.