Anti-Mouse MIP-1γ

Anti-Mouse MIP-1γ

Product No.: M173

[product_table name="All Top" skus="M173"]

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Target
MIP-1 gamma
Product Type
Polyclonal Antibody
Alternate Names
CCL9, CCF18, Macrophage Inflammatory Protein-Related Protein-2 (MRP2), CCL10, SCYB9
Applications
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WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse MIP-1γ (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Additional Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize CCL9/10/MIP‑1 gamma -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL9/10/MIP‑1 gamma.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Mouse Mouse Macrophage Inflammatory Protein-1 Gamma (MIP-1γ) recognizes Mouse MIP-1γ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Macrophage inflammatory protein 1 gamma (MIP-1 gamma) is a monokine with inflammatory, pyrogenic, and chemokinetic CC properties. MIP-1 gamma plays an important role in the differentiation and survival of osteoclasts.1

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Stashenko, P.et al. (2004) J. Immunol. 173: 2084
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General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.