Anti-Mouse PF4 – Biotin

Anti-Mouse PF4 – Biotin

Product No.: C1514

[product_table name="All Top" skus="C1514"]

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Target
PF-4
Product Type
Polyclonal Antibody
Alternate Names
Platelet Factor-4, Oncostatin A, Ironplact, CXCL4, MGC138298, SCYB4
Applications
ELISA Det
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Mouse CXCL4 (Accession # Q9Z126)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CXCL4 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CXCL4 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL when used in conjunction with PN:C1444 as the capture antibody at 4 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse PF4. The sensitivity of this sandwich ELISA is 4-10 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Platelet Factor 4 (PF4) recognizes Mouse PF4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation.1 PF4 is usually found in a complex with proteoglycan. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3B.2
Platelet factor-4 is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.3 PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin.4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Poncz, M. et al. (1990) Blood 76: 336
2. Romagnani, P. et al. (2003) J Exp Med. 197: 1537
3. Eiseman, R. et al. (1990) Blood 76(2): 336-344.
4. Warkentin, TE. et al. (2007) N. Engl. J. Med. 356: 891
ELISA Det
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.