Anti-Mouse PF4 (Clone 140910)

Anti-Mouse PF4 (Clone 140910)

Product No.: C1444

[product_table name="All Top" skus="C1444"]

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Clone
140910
Target
PF-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Oncostatin A, Ironplact, CXCL4, MGC138298, SCYB4, Platelet Factor-4
Isotype
IgG2b
Applications
ELISA Cap
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse CXCL4
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µl/well. A suitable detection antibody is PN:C1444 at a concentration of approximately 50 ng/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 4 ng/well. A suggested standard for this assay is PN:P127.
Western Blotting: To detect Mouse CXCL4 this monoclonal antibody can be used at a concentration of 2-8 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for rmCXCL4 is approximately 25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Platelet factor 4 (PF4) (Clone 140910) recognizes an epitope on Mouse PF4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation.1 PF4 is usually found in a complex with proteoglycan. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3B.2
Platelet factor-4 is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.3 PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin.4

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Poncz, M. et al. (1990) Blood 76: 336
2. Romagnani, P. et al. (2003) J Exp Med. 197: 1537
3. Warkentin, TE. et al. (2007) N. Engl. J. Med. 356: 891
Elisa Sandwich Protocol
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
C1444
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.