Anti-Mouse PlGF-2 (Clone 62526)
Anti-Mouse PlGF-2 (Clone 62526)
Product No.: P279
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Clone 62526 Target PlGF-2 Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2a Applications B , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse PlGF-2 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831575 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse PlGF-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse PlGF-2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Blocking: n a functional ELISA, 0.015 - 0.06 μg/mL of this antibody will block 50% of the binding of 4 ng/mL of recombinant mouse PlGF-2 to immobilized recombinant human Flt-1/Fc Chimera coated at 1 μg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Placenta Growth Factor 2 (PlGF-2) (Clone 62526) recognizes an epitope on Mouse PlGF-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows no cross-reactivity with rhPlGF or rhVEGF. Background Placenta growth factor 2 (PlGF-2) is an isoform of PlGF1 and a member of the vascular endothelial growth factor (VEGF) family. PlGF-2 has heparin binding properties and Neuropilin-1 is a receptor for PlGF-2. Antigen DetailsPubMed References & Citations1. Neufeld, G. et al. (1998) J Biol Chem. 273: 22272 Technical ProtocolsB  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
