Anti-Mouse TCR β chain (TCR) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Home/Mouse T-Cell Receptor Complex, PhenoCycler®, PhenoCycler® Validated Antibodies/Anti-Mouse TCR β chain (TCR) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Pricing & Details

Product No.T900
Clone
H57-597
Protein
TCR beta
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster
IgG
Applications
IHC FF
,
PhenoCycler®
Prod No.
Size
Price
Avail.
Qty
Add to cart
T900-50 µg
50 µg
$95.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

CODEX® Details

Validation Notes
Clone H57-597 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Concentration
0.5 mg/ml
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This TCR β chain (Clone H57-597) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2894398
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.
Antigen Distribution
TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.
Background
TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3,4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5,6.

Antigen Details

Protein
Ligand/Receptor
Peptide bound-MHC class I and II
Function
Antigen recognition, T cell activation
PubMed
NCBI Gene Bank ID

References & Citations

1. Krijgsman D, et al. (2018) Front Immunol. 9:367
2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62
3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43
4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140
5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94
6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7
IHC
PhenoCycler®

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok