Anti-Mouse TCR β chain (TCR) – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Anti-Mouse TCR β chain (TCR) – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Product No.: T900
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Clone H57-597 Target TCR beta Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TCR-β chain, TCR-β, β-TCR Isotype IgG Applications IHC FF , PhenoCycler® |
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CODEX® DetailsValidation Notes Clone H57-597 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue. Tissue Screened Spleen Tissue Preparation FF (Fresh Frozen) Antibody and Reporter DetailsReactivity Species Mouse Host Species Armenian Hamster Concentration 0.5 mg/ml Immunogen Affinity purified TCR from mouse DO-11.10 cells Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4. Storage and Handling This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Applications and Recommended Usage? Quality Tested by Leinco CODEX® This TCR β chain (Clone H57-597) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml. RRID AB_2894398 Country of Origin USA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells. Antigen Distribution TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells. Background TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3,4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5,6. Antigen DetailsProtein Ligand/Receptor Peptide bound-MHC class I and II Function Antigen recognition, T cell activation PubMed NCBI Gene Bank ID References & Citations1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7 Technical Protocols |
Formats Available
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Prod No. | Description |
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T663 | |
T668 | |
T669 | |
T670 | |
T671 | |
T664 | |
T900 | |
T662 | |
T648 | |
T666 | |
T665 | |
T772 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.