Anti-Mouse TNFα [Clone TN3-19.12] — Alexa Fluor® 488
Anti-Mouse TNFα [Clone TN3-19.12] — Alexa Fluor® 488
Product No.: T618AF
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Immunogen Purified Recombinant Mouse TNF-α Product Concentration 0.2 mg/ml Formulation This Alexa Fluor® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Alexa Fluor® is a trademark of Life Technologies Corporation. State of Matter Liquid Storage and Handling This Alexa Fluor® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2-8°C Wet Ice Excitation Laser Blue Laser (488 nm) Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Applications and Recommended Usage? Quality Tested by Leinco Flow CytometryFor flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone TN3-19.12 recognizes mouse TNFα. Background TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Antigen Distribution TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity References & Citations1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 135 2. Adolf GR et al. (1990) Infec Immun. 58: 3996 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
