Anti-Mouse TNF-α – Purified

Pricing & Details

Product No.T259
Tumor Necrosis Factor-α
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Tumor Necrosis Factor Ligand Superfamily Member 2 (TNFSF2), Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin
Armenian Hamster
Prod No.
Add to cart
T259-100 µg
100 µg
In stock
Min: 1
Step: 1
T259-500 µg
500 µg
In stock
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.


Antibody Details

Product Details

Reactivity Species
Host Species
Armenian Hamster
E. coli-expressed, recombinant mouse TNF-α
Product Concentration
0.5 mg/ml
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2 - 6 µg/ml is recommended.
Western Blotting: Clone TN3-19.12, Anti-Mouse TNF-alpha can be used at a concentration of 0.8 µg/ml for western blotting applications. A suitable secondary antibody is Goat-Anti-Armenian Hamster-HRPO (PN:A128) (See Above Image).
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Clone TN3-19.12 recognizes mouse TNFα.
Antigen Distribution
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.
CD120 can refer to two members of the tumor necrosis factor receptor superfamily- CD120a (TNFR1) or CD120b (TNFR2). CD120a is a 55kD Type I transmembrane protein receptor that binds both TNF-α and TNF-β (LT-α). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or TNF-β trimers is vital for signal transduction, leading to apoptosis, NF-B activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. CD120b is a 75 kD type I transmembrane protein that binds both TNF-α and TNF-β. In conjunction with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or TNF-β trimers is critical for signal transduction that may lead to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. TNF-β (LT-α) is a 25 kD protein that has a significant impact on the maintenance of the immune system including the development of secondary lymphoid organs. TNF-β has dual functions. It may function to prevent growth of cancer cells or it may facilitate the development of tumors. TNF-β is involved in the regulation of cell survival, proliferation, differentiation, and apoptosis and, if unregulated, can result in a constantly active signaling pathway, resulting in uncontrolled cellular growth and creation of tumors. Additionally, TNF-β is involved in innate immune regulation and has been shown to prevent tumor growth and obliterate cancerous cell lines.

Antigen Details

Research Area
Innate Immunity

References & Citations

1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 135 2. Adolf GR et al. (1990) Infec Immun. 58: 3996
Elisa Sandwich Protocol
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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