Anti-Mouse TNFα – DyLight® 488
Anti-Mouse TNFα – DyLight® 488
Product No.: T618
- -
- -
Clone TN3-19.12 Target TNF-α Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin Isotype IgG Applications FC |
- -
- -
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Immunogen Purified Recombinant Mouse TNF-α Product Concentration 0.2 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8 degrees C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (493 nm) RRIDAB_2832065 Applications and Recommended Usage? Quality Tested by Leinco Flow CytometryFor flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume or 100 µl of whole blood. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone TN3-19.12 recognizes mouse TNFα. Antigen Distribution TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells. Background TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity References & Citations1. El-Harith el-HA et al. (2004) Saudi Med J. 25: 135
2. Adolf GR et al. (1990) Infec Immun. 58: 3996 Technical Protocols |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
