Anti-Mouse CD120a (TNFR1) (Clone 47803)

Anti-Mouse CD120a (TNFR1) (Clone 47803)

Product No.: T436

[product_table name="All Top" skus="T436"]

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Clone
47803
Target
TNF RI
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFRSF1A, P55, TBP1, CD120a, FPF, MGC19588, TNF-R, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55-R, p60, Tumor Necrosis Factor Receptor I
Isotype
IgG1
Applications
ELISA Cap
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse TNF RI (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T395.
Western Blotting: To detect Mouse TNF RI this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TNF RI is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T395.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse ttmor Necrosis Factor Receptor I (TNF RI) (Clone 47803) recognizes an epitope on Mouse TNF RI. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNF-alpha are mediated.1 TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.2 It regulates immune and inflammatory responses.3

Antigen Details

PubMed

References & Citations

1. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666
2. Donner, DB. et al. (2008) J Immunol. 181: 1288
3. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015
Elisa Sandwich Protocol
General Western Blot Protocol
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.