Anti-Mouse VE-Cadherin
Anti-Mouse VE-Cadherin
Product No.: V172
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Clone 162709 Target VE-Cadherin Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Vascular endothelial-Cadherin Isotype IgG2b Applications FC , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse VE-Cadherin (>98%) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832170 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing VE-Cadherin. A suggested method would be to stain cells expressing VE-Cadherin with 0.25 µg/106 cells in a total staining volume of 200 µl followed by PN:R485. Western Blotting: To detect Mouse VE-Cadherin this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse VE-Cadherin is 5 - 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Vascular endothelial-Cadherin (VE-Cadherin) (Clone 162709) recognizes an epitope on Mouse VE-Cadherin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen DetailsNCBI Gene Bank ID UniProt.org Technical Protocols  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
