Anti-Mouse Vγ 3 (TCR) [Clone 536] — Alexa Fluor® 488

Purified Recombinant Mouse TCR (>98%)

This Alexa Fluor® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

This Alexa Fluor® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Blue Laser (488 nm) Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Syrian Hamster Anti-Mouse Vγ 3 T-Cell Receptor (TCR) (Clone 536) recognizes an epitope on Mouse TCR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

The antibody 536 stained less than 1% of cells in adult thymus (AKR), splenocytes, lymph node, adult CD4-8- thymocytes, and adult CD4-8-, T splenocyte. 92% of day 14 old BALB/C fetal thymus, 86% of C57BL/6 dEC (Thy-1+) and 94% of AKR dEC (Thy-1+) stained positive using this antibody.

1. Havran, et al. (1989) Pro. Natl. Acad. Sci. USA 86:4185