Anti-Mouse Tie-2 – Biotin

Anti-Mouse Tie-2 – Biotin

Product No.: T503

[product_table name="All Top" skus="T503"]

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Target
Tie-2
Product Type
Polyclonal Antibody
Alternate Names
Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domain 2, VMCM1, CD202b, TEK
Applications
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse Tie-2 (Accession # CAA47857)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse Tie-2 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Tie-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T409.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domain 2 (Tie-2) recognizes Mouse Tie-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Tyrosine kinase with immunoglobulin-like and EGF-like domains 2 (Tie-2) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.1 They are expressed almost exclusively in endothelial cells.2 Tie-2 is functional in pericytes and may play an important role in the progression of diabetic retinopathy, by regulating pericyte loss and influencing the activation state and recruitment of pericytes.3 Tie2 has been shown to be up-regulated in psoriasis and in breast cancer tissue and blocking Tie2 action inhibits tumor angiogenesis and tumor growth.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272
2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704
3. Boulton, ME. et al. (2008) Invest Ophthalmol Vis Sci 49: 2163
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.