Anti-Mre11 [12D7]

Product No.: 56211


Clone 12D7 Target Mre11 Formats AvailableView All Product Type Monoclonal Alternate Names EC 3.1.-.-, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A Isotype Mouse IgG1 Applications ELISA , FA , ICC , IF , IHC FFPE , IP , WB


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Data

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (56211) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mre11 antibody [12D7] detects Mre11 protein by western blot analysis. A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human Mre11-transfected 293T cells 7.5% SDS-PAGE Mre11 antibody [12D7] (56211) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (56211) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Mre11 stained by Mre11 antibody [12D7] (56211) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [12D7] (56211) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Mre11 antibody [12D7] detects Mre11 protein at nucleus by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human colon cancer. Mre11 stained by Mre11 antibody [12D7] (56211) diluted at 1:300. Antigen Retrieval: EDTA buffer, 20 min


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen GST fusion protein corresponding to aa 182-582 of Mre11. Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: Use at 0.5-2ug/mL. A band of ~80kDa is detected. Detection of Mre11 in 30ug of whole cell extracts. Immunofluorescence: use at 1-5ug/mL. Detection of Mre11 in HeLa cells fixed in 4% paraformaldehyde for 15 minutes. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity This antibody recognizes human, mouse, and rat Mre11. Background Meiotic recombination 11 (Mre11), a human ortholog of Mre11A, plays an important role in repairing damaged DNA by cleaving broken ends and by providing a platform for other DNA repair proteins. During DNA double-strand break (DSB) repair, Mre11 participates in the amplification of apoptosis signaling if irreversible damage appears. Similarly, programmed DSB is required to initiate homologous recombination during the meiosis process in which MRE11 serves an essential role for DSB repair. Various Mre11 mutations have been identified in several types of cancer. Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:29670289, PubMed:30612738, PubMed:9590181, PubMed:9705271}. NCBI Gene Bank ID MRE11 UniProt.org P49959 Research Area Cancer Research References & Citations View product citations for antibody 56211 on CiteAb Technical Protocols FA ICC IF IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

GST fusion protein corresponding to aa 182-582 of Mre11.

Product Concentration

Lot Specific

Formulation

PBS, pH 7.4.

State of Matter

Liquid

Product Preparation

Purified by Protein G affinity chromatography

Storage and Handling

This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles.

Regulatory Status

Research Use Only

Country of Origin

USA

Shipping

Next Day 2-8°C

Applications and Recommended Usage?
Quality Tested by Leinco

Immunoblotting: Use at 0.5-2ug/mL. A band of ~80kDa is detected. Detection of Mre11 in 30ug of whole cell extracts.

Immunofluorescence: use at 1-5ug/mL. Detection of Mre11 in HeLa cells fixed in 4% paraformaldehyde for 15 minutes.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

This antibody recognizes human, mouse, and rat Mre11.

Background

Meiotic recombination 11 (Mre11), a human ortholog of Mre11A, plays an important role in repairing damaged DNA by cleaving broken ends and by providing a platform for other DNA repair proteins. During DNA double-strand break (DSB) repair, Mre11 participates in the amplification of apoptosis signaling if irreversible damage appears. Similarly, programmed DSB is required to initiate homologous recombination during the meiosis process in which MRE11 serves an essential role for DSB repair. Various Mre11 mutations have been identified in several types of cancer.

Function

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:29670289, PubMed:30612738, PubMed:9590181, PubMed:9705271}.

NCBI Gene Bank ID

MRE11

UniProt.org

P49959

Research Area

Cancer Research