Anti-Neuroligin-4 Antibody (56553)
Anti-Neuroligin-4 Antibody (56553)
Product No.: 56553
Data
Western Blot analysis of Rat Brain Membrane showing detection of ~125-130 kDa Neuroligin 4 protein using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone S98-7 (56553). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody (56553) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa.
Immunohistochemistry analysis using Mouse Anti-Neuroligin 4 Monoclonal Antibody, Clone S98-7 (56553). Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehyde-fixed paraffin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody (56553) at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000. Courtesy of: Rachel Reith, NIH/NIMH. - -
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Antibody DetailsProduct DetailsReactive Species Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 782-945 (intracellular C-terminus) of mouse Neuroligin-4. Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This product is stable for at least one (1) year at -20°C. Regulatory Status For in vitro investigational use only. Not intended for therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-2ug/mL. A band of ~125-130kDa is detected.
Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes mouse Neuroligin-4. It does not cross-react with other
Neuroligins. Background Neuroligin-4 is a neuronal cell surface protein and a member of the type-B carboxylesterase- lipase protein family. Members of this family may act as splice site-specific ligands for beta- neurexins and may be involved in the formation and remodeling of central nervous system synapses. Neuroligin-4 interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants. Function Cell surface protein involved in cell-cell-interactions. Plays a role in the formation or maintenance of synaptic junctions via its interactions (via the extracellular domains) with neurexin family members. Plays a role in synaptic signal transmission. {PubMed:18434543, PubMed:21282647}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical ProtocolsICC IF   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
