Anti-PCNA – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Clone PC10 has been validated for use on CODEX® using human breast cancer Formalin-fixed paraffin-embedded (FFPE) tissue.

Rat PCNA made in the protein A vector pR1T2T

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

AB_2894158

Clone PC10 recognizes an epitope on mouse PCNA.

PCNA is a ubiquitous nuclear protein present in all cell cycle stages, with the highest expression in the early S phase. Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both.

PCNA antibody, clone PC10, recognizes proliferating cell nuclear antigen (PCNA), also known as cyclin, a 36 kDa nuclear protein belonging to the DNA sliding clamp family. PCNA is a ring-shaped homotrimer that encircles and slides along DNA^1,2. PCNA is an auxiliary protein of DNA polymerase δ and ε and tethers the polymerase catalytic unit to the DNA template for efficient DNA synthesis^3-6. PCNA also contributes to chromatin remodeling, DNA repair, sister chromatid cohesion, and cell cycle control⁷. Expression levels of PCNA are associated with proliferation and transformation, and PCNA is commonly used as a marker for proliferating cells^8,9. Antibodies to PCNA are detected in sera from patients with systemic lupus erythematosus (SLE)¹⁰. In addition, PCNA is a reliable diagnostic and prognostic biomarker for cancer, as tumor cells express high levels of PCNA¹¹.

Ubiquitination, Sumoylation

Increases DNA polymerase δ processibility during elongation of the leading strand.

1. Krishna TS, et al. (1994) Cell. 79(7):1233-43
2. Schurtenberger P, et al. (1998) J Mol Biol. 275(1):123-32
3. Kelman, Z. (1997) Oncogene. 14, 629–640
4. Tan CK, et al. (1986) J Biol Chem. 261(26):12310-6
5. Bravo R, et al. (1987) Nature. 8;326(6112):515-7
6. Prelich G, et al. (1987) Nature. 8;326(6112):517-20
7. Maga G, Hubscher U. (2003) J Cell Sci. 116(Pt 15):3051-60
8. Bravo R, et al. (1982) Prog Clin Biol Res. 85 Pt A:235-48.
9. Celis JE, et al. (1984) Leuk Res. 8(2):143-57
10. Miyachi K, et al. (1978) J Immunol. 121(6):2228-34.
11. Naryzhny SN, Lee H. (2007) FEBS Lett.581(25):4917-20