Anti-PCNA – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-PCNA – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: P500

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Clone
PC10
Target
PCNA
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Isotype
IgG2a κ
Applications
IHC FFPE
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone PC10 has been validated for use on CODEX® using human breast cancer Formalin-fixed paraffin-embedded (FFPE) tissue.
Tissue Screened
Breast Cancer
Tissue Preparation
FFPE (Formalin-Fixed Paraffin-Embedded)

Antibody and Reporter Details

Reactivity Species
Rat
Host Species
Mouse
Concentration
0.5 mg/ml
Immunogen
Rat PCNA made in the protein A vector pR1T2T
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This PCNA (Clone PC10) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2894158
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone PC10 recognizes an epitope on mouse PCNA.
Antigen Distribution
PCNA is a ubiquitous nuclear protein present in all cell cycle stages, with the highest expression in the early S phase. Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both.
Background
PCNA antibody, clone PC10, recognizes proliferating cell nuclear antigen (PCNA), also known as cyclin, a 36 kDa nuclear protein belonging to the DNA sliding clamp family. PCNA is a ring-shaped homotrimer that encircles and slides along DNA1,2. PCNA is an auxiliary protein of DNA polymerase δ and ε and tethers the polymerase catalytic unit to the DNA template for efficient DNA synthesis3-6. PCNA also contributes to chromatin remodeling, DNA repair, sister chromatid cohesion, and cell cycle control7. Expression levels of PCNA are associated with proliferation and transformation, and PCNA is commonly used as a marker for proliferating cells8,9. Antibodies to PCNA are detected in sera from patients with systemic lupus erythematosus (SLE)10. In addition, PCNA is a reliable diagnostic and prognostic biomarker for cancer, as tumor cells express high levels of PCNA11.

Antigen Details

Protein
Ligand/Receptor
Ubiquitination, Sumoylation
Function
Increases DNA polymerase δ processibility during elongation of the leading strand.
PubMed
NCBI Gene Bank ID

References & Citations

1. Krishna TS, et al. (1994) Cell. 79(7):1233-43
2. Schurtenberger P, et al. (1998) J Mol Biol. 275(1):123-32
3. Kelman, Z. (1997) Oncogene. 14, 629–640
4. Tan CK, et al. (1986) J Biol Chem. 261(26):12310-6
5. Bravo R, et al. (1987) Nature. 8;326(6112):515-7
6. Prelich G, et al. (1987) Nature. 8;326(6112):517-20
7. Maga G, Hubscher U. (2003) J Cell Sci. 116(Pt 15):3051-60
8. Bravo R, et al. (1982) Prog Clin Biol Res. 85 Pt A:235-48.
9. Celis JE, et al. (1984) Leuk Res. 8(2):143-57
10. Miyachi K, et al. (1978) J Immunol. 121(6):2228-34.
11. Naryzhny SN, Lee H. (2007) FEBS Lett.581(25):4917-20
IHC
PhenoCycler®

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.