Rat PCNA made in the protein A vector pR1T2T
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this anti-PCNA (Clone PC-10) antibody for staining cells in flow cytometry is ≤ 0.125 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this anti-PCNA (Clone PC-10) antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
Additional Reported Applications For Relevant Conjugates ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone PC10 recognizes an epitope on mouse PCNA.
PCNA is a ubiquitous nuclear protein present in all cell cycle stages, with the highest expression in the early S phase. Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both.
PCNA antibody, clone PC10, recognizes proliferating cell nuclear antigen (PCNA), also known as cyclin, a 36 kDa nuclear protein belonging to the DNA sliding clamp family. PCNA is a ring-shaped homotrimer that encircles and slides along DNA1,2. PCNA is an auxiliary protein of DNA polymerase δ and ε and tethers the polymerase catalytic unit to the DNA template for efficient DNA synthesis3-6. PCNA also contributes to chromatin remodeling, DNA repair, sister chromatid cohesion, and cell cycle control7. Expression levels of PCNA are associated with proliferation and transformation, and PCNA is commonly used as a marker for proliferating cells8,9. Antibodies to PCNA are detected in sera from patients with systemic lupus erythematosus (SLE)10. In addition, PCNA is a reliable diagnostic and prognostic biomarker for cancer, as tumor cells express high levels of PCNA11.
Increases DNA polymerase δ processibility during elongation of the leading strand.
NCBI Gene Bank ID
Neuroscience Cell Markers
References & Citations
1. Krishna TS, et al. (1994) Cell. 79(7):1233-43
2. Schurtenberger P, et al. (1998) J Mol Biol. 275(1):123-32
3. Kelman, Z. (1997) Oncogene. 14, 629–640
4. Tan CK, et al. (1986) J Biol Chem. 261(26):12310-6
5. Bravo R, et al. (1987) Nature. 8;326(6112):515-7
6. Prelich G, et al. (1987) Nature. 8;326(6112):517-20
7. Maga G, Hubscher U. (2003) J Cell Sci. 116(Pt 15):3051-60
8. Bravo R, et al. (1982) Prog Clin Biol Res. 85 Pt A:235-48.
9. Celis JE, et al. (1984) Leuk Res. 8(2):143-57
10. Miyachi K, et al. (1978) J Immunol. 121(6):2228-34.
11. Naryzhny SN, Lee H. (2007) FEBS Lett.581(25):4917-20
Products are for research use only. Not for use in diagnostic or therapeutic procedures.