Anti-PCNA – FITC

Pricing & Details

Product No.P105
Clone
PC10
Protein
PCNA
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
Isotype
Mouse
IgG2a κ
Applications
FC
,
ICFC
Prod No.
Size
Price
Avail.
Qty
Add to cart
P105-50 µg
50 µg
$77.00
In stock
Max:
Min: 1
Step: 1
P105-100 µg
100 µg
$148.00
In stock
Max:
Min: 1
Step: 1
P105-200 µg
200 µg
$264.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Rat
Host Species
Mouse
Immunogen
Rat PCNA made in the protein A vector pR1T2T
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Excitation Laser
Blue Laser (490 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this anti-PCNA (Clone PC-10) antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
ICFC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone PC10 recognizes an epitope on mouse PCNA.
Antigen Distribution
PCNA is a ubiquitous nuclear protein present in all cell cycle stages, with the highest expression in the early S phase. Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both.
Background
PCNA antibody, clone PC10, recognizes proliferating cell nuclear antigen (PCNA), also known as cyclin, a 36 kDa nuclear protein belonging to the DNA sliding clamp family. PCNA is a ring-shaped homotrimer that encircles and slides along DNA1,2. PCNA is an auxiliary protein of DNA polymerase δ and ε and tethers the polymerase catalytic unit to the DNA template for efficient DNA synthesis3-6. PCNA also contributes to chromatin remodeling, DNA repair, sister chromatid cohesion, and cell cycle control7. Expression levels of PCNA are associated with proliferation and transformation, and PCNA is commonly used as a marker for proliferating cells8,9. Antibodies to PCNA are detected in sera from patients with systemic lupus erythematosus (SLE)10. In addition, PCNA is a reliable diagnostic and prognostic biomarker for cancer, as tumor cells express high levels of PCNA11.

Antigen Details

Protein
Ligand/Receptor
Ubiquitination, Sumoylation
Function
Increases DNA polymerase δ processibility during elongation of the leading strand.
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Neuroscience
.
Neuroscience Cell Markers
.
Stem Cell

References & Citations

1. Krishna TS, et al. (1994) Cell. 79(7):1233-43
2. Schurtenberger P, et al. (1998) J Mol Biol. 275(1):123-32
3. Kelman, Z. (1997) Oncogene. 14, 629–640
4. Tan CK, et al. (1986) J Biol Chem. 261(26):12310-6
5. Bravo R, et al. (1987) Nature. 8;326(6112):515-7
6. Prelich G, et al. (1987) Nature. 8;326(6112):517-20
7. Maga G, Hubscher U. (2003) J Cell Sci. 116(Pt 15):3051-60
8. Bravo R, et al. (1982) Prog Clin Biol Res. 85 Pt A:235-48.
9. Celis JE, et al. (1984) Leuk Res. 8(2):143-57
10. Miyachi K, et al. (1978) J Immunol. 121(6):2228-34.
11. Naryzhny SN, Lee H. (2007) FEBS Lett.581(25):4917-20
Flow Cytometry

Formats Available