Anti-PfHsp60 Antibody (11146)

Recombinant full-length PfHsp60 (Accession no. XM_001347402.1)

PBS (1.83mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide

Liquid

Purified by Protein A affinity chromatography

This antibody is stable for at least one (1) year at -20°C.

For in vitro investigational use only. Not intended for diagnostic or therapeutic applications.

Next Day 2-8°C

This antibody recognizes P. falciparum Hsp60 and cross-reacts with E.coli Hsp60/GroEl.

Hsp60 is an abundant, constitutively produced heat shock protein that increases in cellular concentration in response to cell shock. It is found in multiple species and is notably homologous in bacteria, plants, and mammals. Hsp60 of Plasmodium falciparum is a polypeptide of 577 amino acids with a calculated molecular weight of 62158 Da. The primary structure of P. falciparum hsp60 contains a putative mitochondrial targeting peptide at its amino- terminus and a GGM motif at its carboxyl- terminus. The overall structure exhibits strong conservation (approximately 50%) to the hsp60 from human and other eukaryotes, but only low homology (< 30%) to a recently reported P. falciparum chaperonin 60 gene. The P. falciparum hsp60 gene is located on chromosome 10. During heat shock, the level of hsp60 transcript in blood stage parasites increases significantly and its accumulation correlates with the duration of the induction.

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.