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CXCL8, MDNCE, Neutrophil Activating Factor (NAF), NAP-1 Fractalkine Mucin-Like Stalk, 3-10C, AMCF-I, GCP-1, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, AP1, SCYB8, TSG-1, B-ENAP, Interleukin-8
Purified Recombinant Porcine IL-8 (>98%)
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Porcine CXCL8 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Porcine CXCL8 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Porcine IL-8/CXCL8 Biotinylated Antibody. Standard: Recombinant Porcine IL-8/CXCL8 Protein.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Porcine CXCL8 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Porcine CXCL8 (at 50 ng/ml) is 3 - 15 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Porcine Interleukin-8 (IL-8) (Clone 105105) recognizes an epitope on Porcine IL-8. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. IL-8 is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Gene of IL-8 is believed to play a role in the pathogenesis of bronchiolitis. While neutrophil granulocytes are the primary target cells of IL-8 there is a relative wide range of cells (endothelial cells, macrophages, mast cells, keratinocytes) responding to this chemokine, too. When first encountering an antigen, the primary cells to encounter it are the macrophages that phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor. Interleukin-8 is often associated with inflammation. As an example, it has been cited as a proinflammatory mediator in psoriasis.1
NCBI Gene Bank ID
References & Citations
1. Haraldsen, G. et al. (1998) J Exp Med. 188: 1751
Products are for research use only. Not for use in diagnostic or therapeutic procedures.