Data
![Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad50 protein stained by Rad50 antibody [13B3] (56213) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-rad50-antibody-56213-1.jpg)
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rad50 protein stained by Rad50 antibody [13B3] (56213) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.![Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. Rad50 antibody [13B3] (56213) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-rad50-antibody-56213-2.jpg)
Rad50 antibody [13B3] detects Rad50 protein at nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
Rad50 antibody [13B3] (56213) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC-3 xenograft. Rad50 antibody [13B3] (56213) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-rad50-antibody-56213-3.jpg)
Rad50 antibody [13B3] detects Rad50 protein at nucleus in PC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
Rad50 antibody [13B3] (56213) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Rad50 stained by Rad50 antibody [13B3] (56213) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-rad50-antibody-56213-4.jpg)
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (56213) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Rad50 stained by Rad50 antibody [13B3] (56213) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-rad50-antibody-56213-5.jpg)
Rad50 antibody [13B3] detects Rad50 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Rad50 stained by Rad50 antibody [13B3] (56213) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (56213) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-rad50-antibody-56213-6.jpg)
HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [13B3] (56213) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. - -
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen GST fusion protein corresponding to the complete coding region (aa 1-425) of Rad50 expressed in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4 State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting,
Immunoprecipitation: use at 0.1-2 ug/mL. Positive control: Raji cells. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human Rad50, a 153 kD protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex. Following DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells. Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:9590181, PubMed:9651580, PubMed:9705271}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & CitationsTechnical ProtocolsICC IF  IHC FFPE  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
