NS0 - Derived Recombinant Rat ALK-7 Extracellular Domain (aa 26 - 108)
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
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Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat ALK-7 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Rat ALK-7 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/mL. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Rat Activin-Receptor-Like Kinase-7 (ALK-7) recognizes Rat ALK-7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Activin receptor-like kinase (ALK) 7 is a type I serine/threonine kinase receptor of the transforming growth factor (TGF)-beta family of proteins.1 ALK7 is most abundantly expressed in human brain, pancreas and colon.2 It negatively regulates glucose-stimulated insulin release in β-cells3 and plays an important role in regulating the functional plasticity of pancreatic islets.3 ALK7 also has an important role in the regulation of body fat deposition. These has implications for the development of treatments against diabetes and obesity.3
NCBI Gene Bank ID
References & Citations
1. Kim, SJ. et al. (2004) J Biol Chem. 279: 28458
2. Ritvos, O. et al. (2001) Cytogenet Cell Genet. 95: 157
3. Ibanez, CF. et al. (2008) Proc Natl Acad Sci USA 105: 7246
Products are for research use only. Not for use in diagnostic or therapeutic procedures.