Anti-Rat ALK-7 (Clone 118310)

Anti-Rat ALK-7 (Clone 118310)

Product No.: A379

[product_table name="All Top" skus="A379"]

- -
- -
Clone
118310
Target
ALK-7
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Activin Receptor-Like Kinase 7
Isotype
IgG2a
Applications
WB

- -
- -
Select Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Rat
Host Species
Mouse
Immunogen
NS0-Derived Recombinant Rat ALK-7 Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat ALK-7 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat ALK-7 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Mouse Anti-Rat Activin Receptor-Like Kinase 7 (ALK-7) (Clone 118310) recognizes an epitope on Rat ALK-7. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Activin receptor-like kinase (ALK) 7 is a type I serine/threonine kinase receptor of the transforming growth factor (TGF)-beta family of proteins.1 ALK7 is most abundantly expressed in human brain, pancreas and colon.2 It negatively regulates glucose-stimulated insulin release in β-cells3 and plays an important role in regulating the functional plasticity of pancreatic islets.3 ALK7 also has an important role in the regulation of body fat deposition. These has implications for the development of treatments against diabetes and obesity.3
PubMed
NCBI Gene Bank ID

References & Citations

1. Kim, SJ. et al. (2004) J Biol Chem. 279: 28458
2. Ritvos, O. et al. (2001) Cytogenet Cell Genet. 95: 157
3. Ibanez, CF. et al. (2008) Proc Natl Acad Sci USA 105: 7246
General Western Blot Protocol

Certificate of Analysis

- -
- -

Formats Available

- -
- -
Prod No.
Description
A379
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.