Data
Antibody DetailsProduct DetailsReactivity Species Rat Host Species Mouse Immunogen Purified Recombinant Rat EGF (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830083 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat EGF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat EGF is 25 and 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E188. Other Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Rat EGF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Rat EGF (at 75 ng/ml) is 0.75-3.75 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat Epidermal Growth Factor (EGF) (Clone 420610) recognizes an epitope on Rat EGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Antigen DetailsProtein PubMed NCBI Gene Bank ID UniProt.org Technical Protocols |
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